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osteoblast growth medium ob supplement mix  (PromoCell)


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    Structured Review

    PromoCell osteoblast growth medium ob supplement mix
    (a) Time –dependent increase in cell numbers (ΔN/Δt) in Dulbecco's Minimal Eagle <t>Medium</t> (DMEM), <t>osteoblast</t> proliferation medium (OB), and NeuroBasal medium containing 50 ng/ml nerve <t>growth</t> factor (NB). The peak values of the curves coincide with the time when the cells reached confluence (t k ), culture well area = 2 cm 2 . (b) Expression of osteocalcin in isolated STRO-1 + cells cultured for several weeks in DMEM and OB-medium. RT-PCR was used to detect the mRNA of osteocalcin (OCN); expression was investigated at culture days 7, 14 and 21. (c,d) STRO-1 + cells after four days of induced adipogenic differentiation in adipocyte differentiation medium starting with intracellular lipid formation (white arrows), (c) phase contrast, (d) varel contrast; scale bars: 100 µm. (e,f) STRO-1 + cells after 10 days culture in adipocyte differentiation medium. Cells were fixed, stained for lipid accumulation (Oil Red O) and observed under a light microscope; scale bars: 100 µm.
    Osteoblast Growth Medium Ob Supplement Mix, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/osteoblast growth medium ob supplement mix/product/PromoCell
    Average 96 stars, based on 217 article reviews
    osteoblast growth medium ob supplement mix - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "Localization and Characterization of STRO-1 + Cells in the Deer Pedicle and Regenerating Antler"

    Article Title: Localization and Characterization of STRO-1 + Cells in the Deer Pedicle and Regenerating Antler

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0002064

    (a) Time –dependent increase in cell numbers (ΔN/Δt) in Dulbecco's Minimal Eagle Medium (DMEM), osteoblast proliferation medium (OB), and NeuroBasal medium containing 50 ng/ml nerve growth factor (NB). The peak values of the curves coincide with the time when the cells reached confluence (t k ), culture well area = 2 cm 2 . (b) Expression of osteocalcin in isolated STRO-1 + cells cultured for several weeks in DMEM and OB-medium. RT-PCR was used to detect the mRNA of osteocalcin (OCN); expression was investigated at culture days 7, 14 and 21. (c,d) STRO-1 + cells after four days of induced adipogenic differentiation in adipocyte differentiation medium starting with intracellular lipid formation (white arrows), (c) phase contrast, (d) varel contrast; scale bars: 100 µm. (e,f) STRO-1 + cells after 10 days culture in adipocyte differentiation medium. Cells were fixed, stained for lipid accumulation (Oil Red O) and observed under a light microscope; scale bars: 100 µm.
    Figure Legend Snippet: (a) Time –dependent increase in cell numbers (ΔN/Δt) in Dulbecco's Minimal Eagle Medium (DMEM), osteoblast proliferation medium (OB), and NeuroBasal medium containing 50 ng/ml nerve growth factor (NB). The peak values of the curves coincide with the time when the cells reached confluence (t k ), culture well area = 2 cm 2 . (b) Expression of osteocalcin in isolated STRO-1 + cells cultured for several weeks in DMEM and OB-medium. RT-PCR was used to detect the mRNA of osteocalcin (OCN); expression was investigated at culture days 7, 14 and 21. (c,d) STRO-1 + cells after four days of induced adipogenic differentiation in adipocyte differentiation medium starting with intracellular lipid formation (white arrows), (c) phase contrast, (d) varel contrast; scale bars: 100 µm. (e,f) STRO-1 + cells after 10 days culture in adipocyte differentiation medium. Cells were fixed, stained for lipid accumulation (Oil Red O) and observed under a light microscope; scale bars: 100 µm.

    Techniques Used: Expressing, Isolation, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Staining, Light Microscopy



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    osteoblast growth medium ob supplement mix  (PromoCell)
    96
    PromoCell osteoblast growth medium ob supplement mix
    (a) Time –dependent increase in cell numbers (ΔN/Δt) in Dulbecco's Minimal Eagle <t>Medium</t> (DMEM), <t>osteoblast</t> proliferation medium (OB), and NeuroBasal medium containing 50 ng/ml nerve <t>growth</t> factor (NB). The peak values of the curves coincide with the time when the cells reached confluence (t k ), culture well area = 2 cm 2 . (b) Expression of osteocalcin in isolated STRO-1 + cells cultured for several weeks in DMEM and OB-medium. RT-PCR was used to detect the mRNA of osteocalcin (OCN); expression was investigated at culture days 7, 14 and 21. (c,d) STRO-1 + cells after four days of induced adipogenic differentiation in adipocyte differentiation medium starting with intracellular lipid formation (white arrows), (c) phase contrast, (d) varel contrast; scale bars: 100 µm. (e,f) STRO-1 + cells after 10 days culture in adipocyte differentiation medium. Cells were fixed, stained for lipid accumulation (Oil Red O) and observed under a light microscope; scale bars: 100 µm.
    Osteoblast Growth Medium Ob Supplement Mix, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/osteoblast growth medium ob supplement mix/product/PromoCell
    Average 96 stars, based on 1 article reviews
    osteoblast growth medium ob supplement mix - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    ob growth medium supplement mix  (PromoCell)
    96
    PromoCell ob growth medium supplement mix
    (a) Time –dependent increase in cell numbers (ΔN/Δt) in Dulbecco's Minimal Eagle <t>Medium</t> (DMEM), <t>osteoblast</t> proliferation medium (OB), and NeuroBasal medium containing 50 ng/ml nerve <t>growth</t> factor (NB). The peak values of the curves coincide with the time when the cells reached confluence (t k ), culture well area = 2 cm 2 . (b) Expression of osteocalcin in isolated STRO-1 + cells cultured for several weeks in DMEM and OB-medium. RT-PCR was used to detect the mRNA of osteocalcin (OCN); expression was investigated at culture days 7, 14 and 21. (c,d) STRO-1 + cells after four days of induced adipogenic differentiation in adipocyte differentiation medium starting with intracellular lipid formation (white arrows), (c) phase contrast, (d) varel contrast; scale bars: 100 µm. (e,f) STRO-1 + cells after 10 days culture in adipocyte differentiation medium. Cells were fixed, stained for lipid accumulation (Oil Red O) and observed under a light microscope; scale bars: 100 µm.
    Ob Growth Medium Supplement Mix, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ob growth medium supplement mix/product/PromoCell
    Average 96 stars, based on 1 article reviews
    ob growth medium supplement mix - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (a) Time –dependent increase in cell numbers (ΔN/Δt) in Dulbecco's Minimal Eagle Medium (DMEM), osteoblast proliferation medium (OB), and NeuroBasal medium containing 50 ng/ml nerve growth factor (NB). The peak values of the curves coincide with the time when the cells reached confluence (t k ), culture well area = 2 cm 2 . (b) Expression of osteocalcin in isolated STRO-1 + cells cultured for several weeks in DMEM and OB-medium. RT-PCR was used to detect the mRNA of osteocalcin (OCN); expression was investigated at culture days 7, 14 and 21. (c,d) STRO-1 + cells after four days of induced adipogenic differentiation in adipocyte differentiation medium starting with intracellular lipid formation (white arrows), (c) phase contrast, (d) varel contrast; scale bars: 100 µm. (e,f) STRO-1 + cells after 10 days culture in adipocyte differentiation medium. Cells were fixed, stained for lipid accumulation (Oil Red O) and observed under a light microscope; scale bars: 100 µm.

    Journal: PLoS ONE

    Article Title: Localization and Characterization of STRO-1 + Cells in the Deer Pedicle and Regenerating Antler

    doi: 10.1371/journal.pone.0002064

    Figure Lengend Snippet: (a) Time –dependent increase in cell numbers (ΔN/Δt) in Dulbecco's Minimal Eagle Medium (DMEM), osteoblast proliferation medium (OB), and NeuroBasal medium containing 50 ng/ml nerve growth factor (NB). The peak values of the curves coincide with the time when the cells reached confluence (t k ), culture well area = 2 cm 2 . (b) Expression of osteocalcin in isolated STRO-1 + cells cultured for several weeks in DMEM and OB-medium. RT-PCR was used to detect the mRNA of osteocalcin (OCN); expression was investigated at culture days 7, 14 and 21. (c,d) STRO-1 + cells after four days of induced adipogenic differentiation in adipocyte differentiation medium starting with intracellular lipid formation (white arrows), (c) phase contrast, (d) varel contrast; scale bars: 100 µm. (e,f) STRO-1 + cells after 10 days culture in adipocyte differentiation medium. Cells were fixed, stained for lipid accumulation (Oil Red O) and observed under a light microscope; scale bars: 100 µm.

    Article Snippet: To investigate the influence of different culture media on the proliferation of STRO-1 + cells, we cultivated cells for one month in Dulbecco's minimal eagle medium [DMEM (Gibco) + 10% FCS], osteoblast growth medium (OB) + supplement mix (both Promocell) or NeuroBasal medium (NB/Gibco) containing 50 ng/ml nerve growth factor (NGF 7S/Invitrogen).

    Techniques: Expressing, Isolation, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Staining, Light Microscopy